nucleic acid/ PCR studies
At the heart of molecular biology is one type of molecule: DNA.
Since the adoption of the Watson-Crick double helix model of DNA, Nucleic Acids have become increasingly important in understanding every living being.
This field has developed very rapidly in the last 65 years. With the milestones of PCR and sequencing, working with DNA and RNA has become one of the most important areas for scientists in the life sciences.
DNA molecules are amplified and introduced into organisms by transformation or transfection, separated, stained, examined under a microscope, manipulated, sequenced, etc.
For all these techniques, the first step is to isolate the DNA from the source of interest.
This page gives you an overview of the different methods for nucleic acid isolation and presents a set of must-have reagents to obtain pure DNA and/or RNA from a variety of sources.
01
DECONTAMINATION
DNA amplification is one of the most commonly used techniques in modern research laboratories. The presence of contaminating DNA in and around the PCR workstation can cause unwanted artifacts during amplification.
Basically, there are two ways to make DNA non-reproducible:
by degradation of DNA (for example, by addition of DNases or chemical degradation), or
by modification of bases - leaving the DNA strand intact, but blocking it for reading by polymerases.
PanReac AppliChem DNA-ExitusPlus™ is a patented reagent for the removal of DNA and RNA contaminations from laboratory surfaces and equipment.
The solution uses mild and non-corrosive chemistry for rapid non-enzymatic degradation of nucleic acids. The already short incubation times with DNA-ExitusPlus™ completely remove unwanted DNA and RNA from work surfaces and instruments.
02
PCR ENZYMES
Proteinase K
RNase A
Lysozyme
DNase I
Taq DNA Polymerase
SuperHot Taq DNA Polymerase
PanReac AppliChem It offers high quality standard enzymes for you to work with nucleic acids. The high level of satisfaction of our customers proves this.
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